50bp DNA Ladder

Concentration: 76ng/µl Recommended Loading 3-5 µl/Lane. Concentration Typical Bands 100 ng/5µl Other Bands 40 ng/5µl Recommended Electrophoresis Condition 5 µl/Lane, 8 cm 3 %Agarose Gel, 0.5×TBE, 7V/cm, 45min. Contents(bp) 50 ,100,150,200,250,300,400,500,Storage1 year at room temperature, for long-time preservation, please storage at -20°C .Description50bp DNA ladder is ideal for determining the size of double-stranded DNA from 50 to 500 base pairs. The ladder consists of 8 linear double-stranded fragments .The 250bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 250bp are 40ng. The 250bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use. RecommendationsDo not heat before loading.For quantification, adjust the concentration of the sample to equalizeit approximately with the amount of DNA in the nearest band of the ladder.Visualize DNA by staining with ethidium bromide or with SYBR® Green I.