1kb DNA Ladder Plus

Concentration: 144ng/µl Recommended Loading 3-5 µl/Lane. Concentration Typical Bands 100 ng/5µl Other Bands 40 ng/5µl Recommended ElectrophoresisCondition 8 cm 5%Agarose Gel, 1×TAE, 5 V/cm, 45min. Contents(bp) 100,200 ,300,400,500,700,1,000, 1,500,2,000,3,000,4,000,5,000,6,000,8,000,10,000Storage1 year at room temperature, for long-time preservation, please storage at -20°C .Description1 kb ladder plus is ideal for determining the size of double-stranded DNA from 100 to 10,000 base pairs. The ladder consists of 15 linear double-stranded fragments .The 500bp and 3,000bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 500bp and 3,000bp are 40ng. The 500bp and 3,000bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use. RecommendationsDo not heat before loading.For quantification, adjust the concentration of the sample to equalizeit approximately with the amount of DNA in the nearest band of the ladder.Visualize DNA by staining with ethidium bromide or with SYBR® Green I.