100bp DNA Ladder

Concentration: 100ng/µl Recommended Loading3-5 µl/Lane. Concentration Typical Bands 100 ng/5µl Other Bands 40 ng/5µlRecommended Electrophoresis Condition 5 µl/Lane, 8 cm 1.7 %Agarose Gel, 0.5×TBE, 7V/cm, 1h. Contents(bp) 100,200,300,400,500,600,700,800,900,1,000,1,500Storage1 year at room temperature, for long-time preservation, please storage at -20°C .Description100bp ladder is ideal for determining the size of double-stranded DNA from 100 to 1500 base pairs. The ladder consists of 11 linear double-stranded fragments .The 500bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 500bp are 40ng. The 500bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use. RecommendationsDo not heat before loading.For quantification, adjust the concentration of the sample to equalizeit approximately with the amount of DNA in the nearest band of the ladder.Visualize DNA by staining with ethidium bromide or with SYBR® Green I.